Correction: A Seroepidemiological Study of Serogroup A Meningococcal Infection in the African Meningitis Belt.
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Published in PLoS ONE
Correction: A Seroepidemiological Study of Serogroup A Meningococcal Infection in the African Meningitis Belt.
...The structure and evolution of centromeric transition regions within the human genome.
...Published in Nature
An understanding of how centromeric transition regions are organized is a critical aspect of chromosome structure and function; however, the sequence context of these regions has been difficult to resolve on the basis of the draft genome sequence. We present a detailed analysis of the structure and assembly of all human pericentromeric regions (5 m...
Investigation of a large community-based outbreak of hepatitis B infection in the United Kingdom.
Published in Epidemiology and Infection
We describe the largest outbreak of hepatitis B virus infection reported to date in the UK. Between July 2001 and December 2005, 237 cases were identified in Avon, South West England. The likely route of transmission was injecting drug use in 44% (104/237) and heterosexual intercourse in 30% (71/237) of cases. A case-control study in injectors show...
Type IV collagens isolation and characterization of 7S collagen from human kidney, liver and lung.
Published in Collagen and Related Research
7S collagens were isolated after bacterial collagenase treatment of basement membrane material prepared from the pepsin digest of human kidney, liver and lung. The 7S collagens were purified by combination of molecular sieve and ion exchange chromatography. 7S collagen from each of these sources showed similar amino acid composition, electrophoreti...
Towards zoomable multidimensional maps of the cell.
Published in Nature Biotechnology
The detailed structure of molecular networks, including their dependence on conditions and time, are now routinely assayed by various experimental techniques. Visualization is a vital aid in integrating and interpreting such data. We describe emerging approaches for representing and visualizing systems data and for achieving semantic zooming, or ch...
Targeted mutagenesis in E. coli: A powerful tool for the generation of random mutant libraries.
Published in Discovery medicine
Extract: Mutagenesis is widely used in the fields of genetics, enzyme catalysis, ligand-binding recognition, metabolic regulation, control of gene expression, and to study mechanisms of DNA repair. Mutations are also introduced to optimize enzyme performance. This area has attracted renewed interest with the increased use of biocatalysts in chemica...
Initial sequencing and analysis of the human genome.
...Published in Nature
The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be...
Application of molecular methods to a nosocomial outbreak of Salmonella enteritidis phage type 4.
Published in Journal of Hospital Infection
A nosocomial outbreak of Salmonella enteritidis phage type 4 occurred in July 1995. Seven definite cases were identified over 13 days affecting four wards in a London hospital. The outbreak strain was characterized by plasmid profile typing and pulsed-field gel electrophoresis (PFGE), and was unusual in that it did not possess a 38 MDa plasmid comm...
Slit2 and netrin 1 act synergistically as adhesive cues to generate tubular bi-layers during ductal morphogenesis.
Published in Development
Development of many organs, including the mammary gland, involves ductal morphogenesis. Mammary ducts are bi-layered tubular structures comprising an outer layer of cap/myoepithelial cells (MECs) and an inner layer of luminal epithelial cells (LECs). Slit2 is expressed by cells in both layers, with secreted SLIT2 broadly distributed throughout the ...
Evolution s cauldron: duplication, deletion, and rearrangement in the mouse and human genomes.
Published in Proceedings of the National Academy of Sciences
This study examines genomic duplications, deletions, and rearrangements that have happened at scales ranging from a single base to complete chromosomes by comparing the mouse and human genomes. From whole-genome sequence alignments, 344 large (>100-kb) blocks of conserved synteny are evident, but these are further fragmented by smaller-scale evolut...
